IN VITRO ORCHID CULTURE MEDIA

VACIN & WENT MINERAL SALT FORMULATION (Russell Modification)


(This formulation does away with the use of the barely soluble Tri-calcium Phosphate for which the original formulation calls, yet gives identical ion concentrations to the original. The Iron-source has additionally been replaced with a commercial chelated form of horticultural iron.)

CALCIUM NITRATE Ca(NO3)2.4H2O 450 mg
POTASSIUM NITRATE KNO3 140 mg
MONO-POTASSIUM PHOSPHATE KH2PO4 80 mg
DI-POTASSIUM PHOSPHATE K2HPO4 440 mg
MAGNESIUM SULPHATE MgSO4.7H2O 250 mg
AMMONIUM SULPHATE (NH4)2SO4 500 mg
MANGANESE SULPHATE MnSO4 7.5 mg
13% IRON CHELATE   40 mg
DISTILLED WATER   to 1 litre


MOTHER FLASK MEDIUM - FOR GERMINATION OF SEED

V & W SALTS    
SUCROSE   20 g
AGAR   8 g
 pH ADJUSTED TO Ca. 6.2 WITH CITRIC ACID (Ca. 75 mg) OR LEMON JUICE (Ca. 1 ml)

GROWTH MEDIUM - FOR REFLASKING SEEDLINGS

V & W SALTS    
SUCROSE   20 g
AGAR   6 g
PEPTONE   1 g
BANANA   Ca. 50 g
NAA   1 mg
 pH ADJUSTED AS ABOVE

SIMPLE MEDIUM

CHEMICULT HYDROPONIC FERTILIZER   2 g
SUCROSE (TABLE SUGAR)   20 g
AGAR   8 g
DISTILLED WATER   to 1 litre


ABOVE MEDIA ARE SUITABLE FOR EPIPHYTIC AND SEMI-EPIPHYTIC ORCHIDS, SUCH AS ANSELLIA, CYMBIDIUM, CATTLEYA, DENDROBIUM, ONCIDIUM, etc. FOR GENERA LIKE EULOPHIA, PAPHIOPEDILUM, THE DISA GROUP, etc., MORE COMPLICATED MEDIA HAVE TO BE USED.

© GREIG RUSSELL 7.11.92

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